To see the issue content and the abstract you do not have to login
Please login to download the full articles

Emailadres
Password
Lost password?      Click here!

If you do not have a subscription to Rhinology please consider to take one.
Click here to become a member of the European Rhinologic Society and a subscriber to the journal `RHINOLOGY`, beginning 2018. Subscription including membership fee: Euro 139.-

If you only want to buy this paper please click here
The price of the paper is 25 euro.


TSLP regulates eotaxin-1 production by nasal epithelial cells from patients with eosinophilic CRSwNP

Volume: 56 - Issue: 4

Firstpage: 370 - Lastpage: 377

W.W. Wang - D.M. Lu - M. Zheng - J.G. Zhang - B. Zhang

BACKGROUND: Eosinophilic chronic rhinosinusitis with nasal polyps (eCRSwNP) is characterized by Th2-skewed inflammation with eosinophilic infiltration. Thymic stromal lymphopoietin (TSLP) promotes the development of allergic inflammation. Although increased TSLP is found in eCRSwNP, little is known about whether TSLP regulates eotaxin-1 production, a potent eosinophil chemoattractant that recruits and activates eosinophils.
Objective: The aim of this study was to investigate the effects and mechanisms of TSLP in eotaxin-1 production in the eosinophilic inflammation of eCRSwNP.
METHODS: Human nasal epithelial cells (HNECs) from eCRSwNP patients were stimulated with recombinant human TSLP in the presence or absence of CYT387 (Janus kinase 1/2 inhibitor). Phosphorylated signal transducer activator of transcription 3 (p-STAT3) was measured by using immunocytochemistry. Eotaxin-1 expression was determined by using real-time PCR. Western blotting was used to detect the levels of p-STAT3 and eotaxin-1 protein.
RESULTS: The treatment with TSLP induced STAT3 phosphorylation in HNECs, and promoted p-STAT3 nuclear translocation, leading to a time-dependent increase of eotaxin-1 expression. However, these effects were attenuated by CYT387 pretreatment.
CONCLUSION: TSLP regulated eotaxin-1 production in HNECs via JAK1/2-STAT3 signaling, which might contribute to the eosinophilic inflammation of eCRSwNP.

W.W. Wang - D.M. Lu - M. Zheng - J.G. Zhang - B. Zhang - TSLP regulates eotaxin-1 production by nasal epithelial cells from patients with eosinophilic CRSwNP
Rhinology 56-4: 370-377, 2018